https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:49869 Wed 07 Jun 2023 15:54:43 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34255 Ex situ analyses of human myometrial tissue has been used to investigate the regulation of uterine quiescence and transition to a contractile phenotype. Following concerns about the validity of cultured primary cells, we examined whether myometrial tissue undergoes culture-induced changes ex situ that may affect the validity of in vitro models. Objectives: To determine whether human myometrial tissue undergoes culture-induced changes ex situ in Estrogen receptor 1 (ESR1), Prostaglandin-endoperoxide synthase 2 (PTGS2) and Oxytocin receptor (OXTR) expression. Additionally, to determine whether culture conditions approaching the in vivo environment influence the expression of these key genes. Methods: Term non-laboring human myometrial tissues were cultured in the presence of specific treatments, including; serum supplementation, progesterone and estrogen, cAMP, PMA, stretch or NF-κB inhibitors. ESR1, PTGS2 and OXTR mRNA abundance after 48 h culture was determined using quantitative RT-PCR. Results: Myometrial tissue in culture exhibited culture-induced up-regulation of ESR1 and PTGS2 and down-regulation of OXTR mRNA expression. Progesterone prevented culture-induced increase in ESR1 expression. Estrogen further up-regulated PTGS2 expression. Stretch had no direct effect, but blocked the effects of progesterone and estrogen on ESR1 and PTGS2 expression. cAMP had no effect whereas PMA further up-regulated PTGS2 expression and prevented decline of OXTR expression. Conclusion: Human myometrial tissue in culture undergoes culture-induced gene expression changes consistent with transition toward a laboring phenotype. Changes in ESR1, PTGS2 and OXTR expression could not be controlled simultaneously. Until optimal culture conditions are determined, results of in vitro experiments with myometrial tissues should be interpreted with caution.]]> Wed 04 Sep 2019 10:05:05 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:32097 Thu 28 Oct 2021 12:37:05 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:8305 Sat 24 Mar 2018 08:40:32 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:13869 Sat 24 Mar 2018 08:25:48 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:21234 Mon 23 Sep 2019 13:08:04 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:32077 0.05). Global DNA methylation levels in males were significantly higher than in females (median %5-mC: 1.82 vs. 1.03, males and females respectively, (P < 0.05)). Conclusion: No association was found between the intake of one-carbon metabolism nutrients during the early postnatal period and global DNA methylation levels at age four years. Higher global DNA methylation levels in males warrants further investigation.]]> Mon 23 Sep 2019 11:18:49 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:54746 Mon 11 Mar 2024 14:25:24 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:40011 p > .05), though the estimates of effect were consistently negative. Global DNA methylation levels in males were significantly higher than in females (median %5mC: 1.82 vs. 1.03, males and females, respectively, (p < .05)). Conclusion: No association was found between global DNA methylation and child cognition and behavior; however given the small sample, this study should be pooled with other cohorts in future meta-analyses.]]> Fri 15 Jul 2022 10:09:55 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:32098 Fri 03 Dec 2021 10:35:09 AEDT ]]>